Molecular Biology Insights Oligo 7.57 | 13.4 MB
OLIGO
Primer Analysis Software is the essential tool for designing and
analyzing sequencing and PCR primers, synthetic genes, and various kinds
of probes including siRNA and molecular beacons. Based on the most
up-to date nearest neighbor thermodynamic data, Oligo's search
algorithms find optimal primers for PCR, including TaqMan, highly
multiplexed, consensus or degenerate primers. Multiple file batch
processing is possible. It is also an invaluable tool for site directed
mutagenesis.
For each primer or primer pair, Oligo's various
analysis windows show a multitude of useful data, such as DNA and RNA
secondary structure, dimer formation, false priming and homology,
internal stability, composition and physical properties. With Oligo you
can analyze open reading frames down to predicted molecular weight and
pKa of proteins, and search for restriction enzyme sites, not only in
DNA but also in reverse-translated proteins.
The first version of
the software appeared on the market in 1989. It went through several
modifications, and the last one, the change from version 6 to 7, was the
most comprehensive. Oligo 7 can automatically select multiplex primers,
process sequence files in batch modes, automatically design PCR primers
to cover multiple DNA regions in just one search and automatically find
primer/probe sets for real time PCR or finds nested primers sets. Oligo
search protocols (scoring system) may be customized in detail, so you
may optimize the results according to your specific needs.
Oligo
runs on Macintosh and Windows and it may be downloaded from this site.
Click on the Tutorials link above to see Oligo 7 general overview and
the major windows screen designs.
With this version you may:
- analyze your own primers (Tm, dimers, secondary structure, internal stability & more)
- multiplex your sets of primers & probes
- check functionality of the software based on a pre-loaded sequence.
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